Use of community genome arrays (CGAs) to assess the effects of Acacia angustissima on rumen ecology.
نویسندگان
چکیده
This research developed a community genome array (CGA) to assess the effects of Acacia angustissima on rumen microbiology. A. angustissima produces non-protein amino acids as well as tannins, which may be toxic to animals, and CGA was used to assess the effects of this plant on the ecology of the rumen. CGAs were developed using a 7.5 cmx2.5 cm nylon membrane format that included up to 96 bacterial genomes. It was possible to separately hybridize large numbers of membranes at once using this mini-membrane format. Pair-wise cross-hybridization experiments were conducted to determine the degree of cross-hybridization between strains; cross-hybridization occurred between strains of the same species, but little cross-reactivity was observed among different species. CGAs were successfully used to survey the microbial communities of animals consuming an A. angustissima containing diet but quantification was not precise. To properly quantify and validate the CGA, Fibrobacter and Ruminococcus populations were independently assessed using 16S rDNA probes to extracted rRNA. The CGA detected an increase in these populations as acacia increased in the diet, which was confirmed by rRNA analysis. There was a great deal of variation among strains of the same species in how they responded to A. angustissima. However, in general Selenomonas strains tended to be resistant to the tannins in the acacia while Butyrivibrio fibrisolvens was sensitive. On the other hand some species, like streptococci, varied. Streptococcus bovis-like strains were sensitive to an increase in acacia in the diet while Streptococcus gallolyticus-like strains were resistant. Strep. gallolyticus has independently been shown to be resistant to tannins. It is concluded that there is significant variation in tannin resistance between strains of the same species. This implies that there are specific molecular mechanisms at play that are independent of the phylogenetic position of the organism.
منابع مشابه
The Effects of Use Medicinal Plants on Rumen Fermentation Parameters in Ruminants
Rumen is a persistent and specific ecosystem consists of bacteria, protozoa and fungus where feed fermentation takes place in it. Produced Hydrogen in rumen can be used in the synthesis of the volatile fatty acids and the microbial protein and its excess would be eliminated through the production of Methane by methanogenesis. Nutritionists have tried to find ways to decrease loss and energy and...
متن کاملRumen Microbial Community of Saanen Goats Adapted to a High-Fiber Diet in the Northeast of Iran
Swiss Saanen goat is a widespread breed frequently found in commercial herds across the world. The present study aimed to identify the rumen microbial community of exotic Saanen goats adapted to a fibrous diet using barcoded pyrosequencing. Rumen content samples were collected from the four animals via a stomach tube after the morning graze and freeze-dried for DNA extraction. Bacterial and arc...
متن کاملThe Effects of Use Medicinal Plants on Rumen Fermentation Parameters in Ruminants
Rumen is a persistent and specific ecosystem consists of bacteria, protozoa and fungus where feed fermentation takes place in it. Produced Hydrogen in rumen can be used in the synthesis of the volatile fatty acids and the microbial protein and its excess would be eliminated through the production of Methane by methanogenesis. Nutritionists have tried to find ways to decrease loss and energy and...
متن کاملPatch Enclosure and Localized Effects of Selected Acacia Species on Herbaceous Richness and Soil Properties of Rangelands in Somali Regional State in Ethiopia
Enclosure and Acacia shade availability to plants are basic variable in arid and semi-arid rangelands. the aim of this study was investigation the impact of patch enclosure and Acacia shade using four treatments which are Inside Enclosure Under Acacia shade (IEUA), Inside Enclosure Without Acacia shade (IEWA), Outside Enclosure Under Acacia shade (OEUA) and Outside Enclosure W...
متن کاملEnsifer mexicanus sp. nov. a new species nodulating Acacia angustissima (Mill.) Kuntze in Mexico.
A new lineage of Ensifer nodulating the American legume Acacia angustissima in the tropical forest of Chiapas and Morelos, Mexico is described. Bacteria were identified as Ensifer with ssb or nolR specific primers. Phylogenetic analysis with partial sequences of the five chromosomal genes gyrA, nolR, recA, rpoB and rrs revealed that this new lineage is related to African Ensifer terangae. The r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Microbiology
دوره 150 Pt 9 شماره
صفحات -
تاریخ انتشار 2004